This study aimed at assessing the effectiveness of work culture on project performance in Non -Governmental Organizations (NGOs) with reference to the Economic Policy Research Network (EPRN Rwanda). It was carried out based on three objectives:(i) To ascertain the effectiveness of technology-driven organization’s work culture on project performance in Non-Government Organizations, (ii)To determine the effectiveness of short-term contract driven organization’s work culture on project performance in Non-Government Organizations, (iii)To assess the effect of Key Performance Indicators (KPI) driven organization’s work culture on project performance in Non-Governmental Organization. This study employed a case study design to examine one case of Economic Policy Network Research (EPNR Rwanda). Data were collected using primary and secondary data. The study adopted descriptive and inferential statistics.  Questionnaires, interviews, and documentary reviews were the three tools for data collection. Secondary sources were analyzed using descriptive statistics as well as multiple regression analysis. The findings showed that a technology-driven organization’s work culture, key performance indicators are driven organization work culture, and short-term contract-driven organization work culture contribute 82.6% to the performance of projects in NGOs in Rwanda. 81.5% strongly agree that technology-driven work culture led to project performance more than physical supervision and 97.6% strongly agree on setting targets and committing to achieving should lead to project performance and about 87.9% strongly agree that KPI driven work culture reduces time wastage and promote effective use of resources while 94.4% strongly agree that KPI driven work culture enhances auto evaluation then accelerate efficiency and minimize supervision. The researcher recommended that; there should be a study aiming at assessing the technology gap in NGOs and monitoring its daily effectiveness. Finally, NGOs should adopt the use of Key performance indicators as monitoring and evaluation strategies to ensure good performance of organization staff.                                                                       

Keywords: Non – Governmental Organization, Work culture, Project performance, EPRN Rwanda